6160-78-7, 4-Methylumbelliferyl β-D-galactopyranoside, CAS: 6160-78-7

4-MU-b-D-Gal
MUGal
, 4-Methylumbelliferyl β-D-galactopyranoside,
CAS: 6160-78-7
C16H18O8 / 338.31
MFCD00036773

4-甲基伞形酮-beta-D半乳糖苷,4-MU-b-D-Gal

4-Methylumbelliferyl b-D-galactopyranoside

Fluorescent substrate for detecting β-galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. This substrate yields a water-soluble blue fluorescent coumarin fluorophore upon cleavage.

4-Methylumbelliferyl-β-D-galactoside (MUG) is a fluorogenic substrate for β-galactosidase (β-gal). Hydrolysis of MUG by β-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm. MUG has been used to detect β-gal activity in intact bacteria, yeast, and mammalian cells.

4-methylumbelliferyl beta-D-galactoside is a beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position. It has a role as a chromogenic compound. It is a member of coumarins, a monosaccharide derivative and a beta-D-galactoside. It derives from a 4-methylumbelliferone.

CAS Number	6160-78-7
Product Name	4-Methylumbelliferyl-galactopyranoside
IUPAC Name	4-methyl-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
Molecular Formula	C16H18O8
Molecular Weight	338.31 g/mol
InChI	InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/m1/s1
InChI Key	YUDPTGPSBJVHCN-DZQJYWQESA-N
SMILES	CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)O)O)O
Synonyms	4-methylumbelliferone-alpha-D-galactoside, 4-methylumbelliferyl galactose, 4-methylumbelliferyl-alpha-D-galactopyranoside, 4-methylumbelliferyl-beta-D-galactopyranoside, 4-methylumbelliferyl-beta-D-galactoside, 4-methylumbelliferyl-galactopyranoside, 4-methylumbelliferyl-galactopyranoside, ((alpha-D)-galactopyranosyloxy)-isomer, 4-methylumbelliferyl-galactopyranoside, ((beta-D)-galactosyloxy)-isomer, 4-MUBF-GP
Canonical SMILES	CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)O)O)O
Isomeric SMILES	CC1=CC(=O)OC2=C1C=CC(=C2)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O

CAS No: 6160-78-7 Synonyms: 4-MU-b-D-GalMUGal MDL No: MFCD00036773 Chemical Formula: C16H18O8 Molecular Weight: 338.31
COA:

Product name: 4-Methylumbelliferyl β-D-galactopyranoside

CAS: 6160-78-7 M.F.: C₁₆H₁₈O₈ M.W._:338.31

*Items*	*Standards*	*Results*
Appearance	White crystal powder	Complies
Solubility	Soluble in DMF, insoluble in ether	Complies
Identification	IR and HPLC	Complies
NMR and MS	Should comply	Complies
Water Content （K.F.）	Max. 1%	0.2%
Heavy metal	Max. 20ppm	Complies
Residue on ignition	Max. 0.5%	0.1%
4-Methylumbelliferone	Max. 0.1%	Complies
TLC	Should be one spot	One spot
Assay by HPLC	Min. 98%	98.8%

References: 1. Rakhmanova VA, MacDonald RC,, Anal. Biochem., 1998, 257, p2342. Vernet M et al., Application of LacZ gene fusions to preimplantation development. Meth. Enzymol. 1993, 225, p434-451

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