a-D-Glucopyranoside-b-D-fructofuranosyl octadecanoate

Quantification of a-D-glucopyranoside-b-D-fructofuranosyl octadecanoate is possible by real-time PCR. The polymerase chain reaction (PCR) technique is used to detect the presence or absence of DNA or RNA. This process starts with the enzyme DNA polymerase and deoxyribonucleotide triphosphates, which use a primer sequence to synthesize complementary strands of DNA from the template. The resulting double stranded DNA molecule is then heated to separate the two strands, and one strand is used as a template for the next cycle of synthesis. Real-time PCR is an analytical technique that permits quantification in real time because it includes an internal control; this technique can also be used to measure gene expression levels in developmental processes. Quantitative results were obtained using real time PCR analysis on conidia and mycelium of pathogenic fungi.