对甲基苯基-beta-D-半乳糖苷,4-Methylphenyl b-D-galactopyranoside

4-Methylphenyl beta-D-galactopyranoside (MPG) is a synthetic compound that is widely used as a biochemical probe to study the activity of galactosidases, enzymes involved in the breakdown of carbohydrates. MPG is a glycoside consisting of a galactose molecule attached to a methylphenyl group through a β-glycosidic linkage.

Definition and Background:

MPG is a synthetic compound used extensively in the field of biochemistry to study the activity of galactosidases. It is used as a substrate to determine the activity of these enzymes, which are involved in the breakdown of carbohydrates in living organisms.

Physical and Chemical Properties:

MPG is a white crystalline powder that is soluble in water and organic solvents. Its chemical formula is C14H20O6, and it has a molecular weight of 284.31 g/mol. It is relatively stable at room temperature and can be stored for extended periods without significant degradation.

Synthesis and Characterization:

MPG can be synthesized using a variety of methods, including chemical synthesis, enzymatic synthesis, and fermentation. The production of MPG involves the condensation of galactose with a methylphenyl group using various chemical reagents, such as sulfuric acid or hydrochloric acid.

Analytical Methods:

The activity of galactosidases can be measured using various analytical methods, including spectrophotometry, HPLC (High-Performance Liquid Chromatography), and mass spectrometry. These methods allow researchers to assess the activity of these enzymes in vitro and in vivo.

Biological Properties:

MPG has been shown to be an effective substrate for galactosidases in various biological systems, including bacteria, plants, and animals. Its ability to interact with these enzymes makes it a valuable tool for studying the activity of galactosidases and their role in carbohydrate metabolism.

Toxicity and Safety in Scientific Experiments:

Few studies have assessed the toxicity of MPG, but it is generally considered to be safe at low concentrations. However, as with all chemicals, precautions should be taken to ensure safe handling.

Applications in Scientific Experiments:

MPG is widely used in scientific experiments to study the activity of galactosidases and their role in carbohydrate metabolism. It is also used as a tool to develop inhibitors of these enzymes, which may have therapeutic potential in the treatment of various diseases.

Current State of Research:

Research on MPG is ongoing, with scientists continuing to investigate its potential applications in various fields of research and industry. Some current areas of focus include the development of new methods for synthesizing MPG and the optimization of analytical methods for measuring galactosidase activity.

Potential Implications in Various Fields of Research and Industry:

MPG has numerous potential implications in various fields of research and industry. It is used in the food industry to produce lactose-free dairy products and may have future applications in the development of drugs for the treatment of diseases such as cancer and lysosomal storage disorders.

Limitations and Future Directions:

There are some limitations to the use of MPG as a biochemical probe, including its relatively low solubility and potential toxicity at high concentrations. Future directions for research may include the development of new substrates for galactosidases, the optimization of analytical methods for measuring enzyme activity, and the development of new inhibitors of these enzymes for the treatment of disease.

Future Directions:

1. Development of new and more effective methods for synthesizing MPG.

2. Investigation of the potential therapeutic applications of MPG in the treatment of diseases such as cancer and lysosomal storage disorders.

3. Development of new inhibitors of galactosidases for therapeutic purposes.

4. Optimization of analytical methods for measuring galactosidase activity.

5. Investigation of the role of galactosidases in carbohydrate metabolism and its implications for human health and disease.

6. Exploration of the potential use of MPG in the production of lactose-free dairy products.

7. Investigation of the potential impacts of MPG on the environment and its interactions with living organisms.

8. Development of MPG analogs with improved solubility and reduced toxicity.

9. Investigation of the potential use of MPG in the field of carbohydrate biochemistry and glycobiology.

10. Exploration of the potential use of MPG in the development of new diagnostic tools for the detection and monitoring of disease.

COA:

Product name: 4-Methylphenyl b-D-galactopyranoside  

CAS: 3150-22-9          Batch No: 20110801         Quantity: 8g