C2FDG;5-Acetamidofluorescein-di-(b-D-galactopyranoside)

2-Acetylaminofluorescein-di-(b-D-galactopyranoside)

5-Acetamidofluorescein-di-(b-D-galactopyranoside) is a fluorescent compound that is widely used as a reporter in transcriptional regulation studies. It can be detected with a luciferase assay, which makes it useful for diagnostic purposes. 5-Acetamidofluorescein-di-(b-D-galactopyranoside) is also used to measure bacterial enzyme activities and has been localized in host plants. This product is ideal for assays of biological samples, such as tuberculosis or bacterial enzymes. 5-Acetamidofluorescein-di-(b-D-galactopyranoside) can be used in vitro to measure the transcriptional activity of cells.

5-Acetamidofluorescein-di-(b-D-galactopyranoside) is a chemical compound that is commonly used in scientific experiments due to its unique properties. This compound is composed of two components: the fluorophore 5-acetamidofluorescein and the acceptor molecule β-D-galactopyranoside. The combination of these two components creates a sensitive and specific probe that can be used in the detection of specific enzymes, such as β-galactosidase. In this paper, we will explore the definition, physical and chemical properties, synthesis and characterization, analytical methods, biological properties, toxicity and safety in scientific experiments, applications in scientific experiments, current state of research, potential implications in various fields of research and industry, limitations, and future directions of 5-Acetamidofluorescein-di-(b-D-galactopyranoside).

Definition and Background:

5-Acetamidofluorescein-di-(b-D-galactopyranoside) is a chemical compound that consists of a fluorophore and a galactopyranoside acceptor molecule. This compound is commonly used in scientific experiments to detect the presence of enzymes that can cleave the galactopyranoside bond, such as β-galactosidase. The cleavage of this bond leads to an increase in fluorescence, which can be detected and measured.

Physical and Chemical Properties:

5-Acetamidofluorescein-di-(b-D-galactopyranoside) is a white to off-white crystalline powder. It has a molecular weight of 759.67 g/mol and a melting point of around 179-182°C. It is soluble in water and dimethyl sulfoxide (DMSO) but insoluble in most organic solvents. The chemical formula of this compound is C₃₃H₃₉NO₁₅.

Synthesis and Characterization:

5-Acetamidofluorescein-di-(b-D-galactopyranoside) can be synthesized by coupling 5-acetamidofluorescein with p-nitrophenyl-β-D-galactopyranoside (PNPG) in the presence of a catalyst, such as 4-dimethylaminopyridine (DMAP). The reaction is typically carried out in a solvent, such as tetrahydrofuran (THF), and the resulting product is purified by chromatography. The compound can be characterized by various techniques, such as nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and high-performance liquid chromatography (HPLC).

Analytical Methods:

There are several analytical methods available for the detection and quantification of 5-Acetamidofluorescein-di-(b-D-galactopyranoside) in scientific experiments. These methods include fluorescence spectroscopy, absorbance spectroscopy, HPLC, and MS. Fluorescence spectroscopy is the most commonly used method for the detection of this compound due to its high sensitivity and specificity.

Biological Properties:

5-Acetamidofluorescein-di-(b-D-galactopyranoside) is commonly used as a substrate for the detection of β-galactosidase activity in biological samples. β-galactosidase is an enzyme that hydrolyzes the galactopyranoside bond in this compound, leading to an increase in fluorescence that can be detected and measured. The compound has also been used in the detection of other enzymes and in various imaging techniques.

Toxicity and Safety in Scientific Experiments:

There is limited information available on the toxicity and safety of 5-Acetamidofluorescein-di-(b-D-galactopyranoside) in scientific experiments. However, this compound is generally considered to be safe for use in laboratory experiments at the recommended concentrations.

Applications in Scientific Experiments:

5-Acetamidofluorescein-di-(b-D-galactopyranoside) has a wide range of applications in scientific experiments. It is commonly used as a substrate for the detection of β-galactosidase activity in biological samples. The compound has also been used in the detection of other enzymes, such as α-fucosidase and hyaluronidase. In addition, it has been used in various imaging techniques, such as confocal microscopy and flow cytometry.

Current State of Research:

There is ongoing research on the use of 5-Acetamidofluorescein-di-(b-D-galactopyranoside) in various fields of research and industry. This compound has been used in the development of diagnostic assays for the detection of specific enzymes in biological samples. It has also been used in the development of imaging techniques for the detection of cancer cells and other diseases.

Potential Implications in Various Fields of Research and Industry:

5-Acetamidofluorescein-di-(b-D-galactopyranoside) has potential implications in various fields of research and industry. It can be used in the development of diagnostic assays for the detection of specific enzymes in biological samples, which can be useful in the diagnosis and treatment of various diseases. In addition, it can be used in the development of imaging techniques for the detection of cancer cells and other diseases.

Limitations and Future Directions:

There are some limitations to the use of 5-Acetamidofluorescein-di-(b-D-galactopyranoside) in scientific experiments, such as its limited stability and potential interference with other molecules in biological samples. In the future, research should focus on improving the stability and specificity of this compound for use in various applications. Future directions may also include the development of novel substrates and probes for the detection of specific enzymes and biomolecules in biological samples.

Future Directions:

1. Development of new substrates and probes with improved stability and specificity for the detection of specific enzymes and biomolecules.

2. Application of 5-Acetamidofluorescein-di-(b-D-galactopyranoside) in the development of new diagnostic assays for the detection of specific enzymes in biological samples.

3. Further research into the use of this compound in imaging techniques for the detection of cancer cells and other diseases.

4. Investigation of the potential applications of 5-Acetamidofluorescein-di-(b-D-galactopyranoside) in the pharmaceutical industry for drug discovery and development.

5. Development of new analytical methods for the detection and quantification of this compound in biological samples.