637-01-4 , Wurster's reagent; TMPPD ; N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride

637-01-4 , Wurster's reagent; TMPPD ;
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride
C10H16N2·2HCl / 237.17
MFCD00012482

Wurster's reagent; TMPPD

N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride

TMPD is an easily oxidizable compound that serves as a reducing co-substrate for heme peroxidases. TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm. Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200. TMPD is also used for the detection of peroxidases on polyacrylamide gels.

Reducing co-substrate for heme peroxidases; test reagent in microbiology

CAS Number	637-01-4
Product Name	N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride
IUPAC Name	1-N,1-N,4-N,4-N-tetramethylbenzene-1,4-diamine;dihydrochloride
Molecular Formula	C10H18Cl2N2
Molecular Weight	200.71 g/mol
InChI	InChI=1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4;;/h5-8H,1-4H3;2*1H
InChI Key	FBHKTSXMTASXFJ-UHFFFAOYSA-N
SMILES	CN(C)C1=CC=C(C=C1)N(C)C.Cl.Cl
Synonyms	Blue, Wurster, Blue, Wurster's, Reagent, Wurster's, Tetramethylphenylenediamine, Wurster Blue, Wurster Reagent, Wurster's Blue, Wurster's Reagent, Wursters Blue, Wursters Reagent
Canonical SMILES	C[NH+](C)C1=CC=C(C=C1)[NH+](C)C.[Cl-].[Cl-]
Isomeric SMILES	C[NH+](C)C1=CC=C(C=C1)[NH+](C)C.[Cl-].[Cl-]