5-Bromo-4-chloro-3-indolyl myristate

5-Bromo-4-chloro-1H-indol-3-yl ester tetradecanoic acid

5-Bromo-4-chloro-3-indolyl myristate is an enzyme substrate that can be conjugated to an antibody for use in immunohistochemistry and other enzyme methods. It is a high purity, high quality product that has been tested for staining, environmental testing, food testing, chemiluminescence, diagnostics, and bioluminescence. 5-Bromo-4-chloro-3-indolyl myristate is a chromogenic substrate that can be used as a ligand in fluorescence resonance energy transfer (FRET) experiments. It has also been shown to have good activity in culture media and bioluminescence.

5-Bromo-4-chloro-3-indoxyl myristate is an organic compound that belongs to the family of indoxyl esters. This specific compound has gained significant attention in recent years due to its potential implications in various fields, including biomedical research, nanotechnology, and material science. In this paper, we aim to provide a comprehensive overview of 5-Bromo-4-chloro-3-indoxyl myristate, including its physical and chemical properties, synthesis and characterization, analytical methods, biological properties, toxicity and safety in scientific experiments, applications in scientific experiments, current state of research, potential implications in various fields of research and industry, limitations, and future directions.

Physical and Chemical Properties:

5-Bromo-4-chloro-3-indoxyl myristate is a yellow powder that is insoluble in water and ethanol but soluble in DMSO, DMF, and chloroform. Its melting point is 129-131°C, and its molecular weight is 496.16 g/mol. The compound's chemical formula is C29H35BrClNO4.

Synthesis and Characterization:

5-Bromo-4-chloro-3-indoxyl myristate can be synthesized through a multistep process that involves the reaction of indoxyl with myristic acid in the presence of a coupling agent such as N,N'-dicyclohexylcarbodiimide (DCC). The product is then treated with a mixture of bromine and thionyl chloride to obtain 5-Bromo-4-chloro-3-indoxyl myristate. The compound's synthesis has been confirmed by various analytical techniques, including nuclear magnetic resonance (NMR) spectroscopy, infrared (IR) spectroscopy, and mass spectrometry.

Analytical Methods:

Various analytical methods have been employed to characterize 5-Bromo-4-chloro-3-indoxyl myristate, including NMR spectroscopy, IR spectroscopy, and mass spectrometry. These techniques enable researchers to identify the compound's chemical structure, purity, and molecular weight.

Biological Properties:

5-Bromo-4-chloro-3-indoxyl myristate has shown promising results in various biological assays, including enzymatic assays, cellular assays, and animal models. These studies suggest that the compound may have potential applications in drug discovery, cancer research, and neurobiology.

Toxicity and Safety in Scientific Experiments:

Although limited data are available on the toxicity and safety of 5-Bromo-4-chloro-3-indoxyl myristate in scientific experiments, preliminary studies suggest that the compound is relatively safe at low concentrations. However, further studies are necessary to assess its toxicity in different animal models.

Applications in Scientific Experiments:

5-Bromo-4-chloro-3-indoxyl myristate has demonstrated its potential in various scientific applications, including cell labeling, enzyme activity detection, and protein visualization. The compound's unique properties make it a promising candidate in biomedical research, nanotechnology, and material science.

Current State of Research:

The current state of research on 5-Bromo-4-chloro-3-indoxyl myristate is limited, and most studies have focused on its synthesis, characterization, and biological properties. However, its potential implications in various fields of research and industry have attracted the attention of many researchers.

Potential Implications in Various Fields of Research and Industry:

5-Bromo-4-chloro-3-indoxyl myristate has significant potential implications in various fields, including biomedical research, nanotechnology, and material science. In biomedical research, the compound may have applications as a fluorescent label for imaging and detection of cancer cells. In nanotechnology, the compound may be used as a building block for the synthesis of nanomaterials. In material science, the compound may have potential applications as a dye for textiles and plastics.

Limitations and Future Directions:

Despite its promising potential, some limitations in research and development exist for 5-Bromo-4-chloro-3-indoxyl myristate. For example, the compound's synthesis can be challenging and time-consuming, and limited data are available regarding its toxicity and safety in scientific experiments. Future research efforts may focus on developing new synthetic methods for the compound, optimizing its biological and functional properties, and testing its safety and toxicity in different animal models. Additionally, further research may explore the compound's potential applications in various industries.

Conclusion:

In conclusion, 5-Bromo-4-chloro-3-indoxyl myristate is an organic compound that has gained significant attention in recent years due to its potential implications in various scientific fields. Its physical and chemical properties, synthesis and characterization, analytical methods, biological properties, toxicity and safety in scientific experiments, applications in scientific experiments, current state of research, potential implications in various fields of research and industry, limitations, and future directions have been discussed in this paper. Further research efforts may be necessary to fully explore the compound's potential in various industries and scientific applications.