Neu5Aca(2-3)Galb(1-4)Glc-b-pNP

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-b-D-glucopyranoside

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-b-D-glucopyranoside is a fluorescent substrate that can be used for the detection of β-galactosidase. It fluoresces when cleaved by β-galactosidase, which is an enzyme that catalyzes the hydrolysis of terminal galactose residues in the glycosidic linkage of β-galactosides. This product is an excellent substrate for use in chemiluminescence and bioluminescence assays. 4NPBG has been used to detect β-galactosidase activity in diverse environments, such as food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, and conjugate. It is also used in diagnostics, ligand binding assays.