Nonyl b-D-thiomaltopyranoside

正壬基-beta-D-1-硫代麦芽糖苷

Nonyl b-D-thiomaltopyranoside is a custom synthesis of an oligosaccharide and polysaccharide. It is prepared by the modification of saccharides with methylation, glycosylation and carbamylation. The compound has CAS No. 148565-55-3 and can be used as a fluorescent probe for carbohydrate binding proteins. Nonyl b-D-thiomaltopyranoside is a high purity synthetic sugar that has been fluorinated. Click modification has been performed on this sugar to create a fluorescent probe for carbohydrate binding proteins. This sugar has also been synthesized using the technique of glycosylation, which involves the addition of monosaccharides to form disaccharides or polysaccharides.

Nonyl-b-D-1-thiomaltoside is a nonionic surfactant that is commonly used in biological research. It is a synthetic detergent that consists of a hydrophobic nonyl group and a hydrophilic maltoside group, with a thiol group at the anomeric carbon of the reducing end of the sugar. This unique structure gives NDTM its ability to solubilize membrane proteins, making it a popular choice for researchers who want to study the structure and function of membrane proteins. NDTM is derived from maltose, a natural sugar that is found in many foods.

Synthesis and Characterization:

NDTM can be synthesized using a variety of different methods, but the most common approach is the one-pot synthesis of thiomaltosides using NBD-Cl. This method involves the condensation of maltose with NBD-Cl in the presence of a base, followed by thiolysis of the resulting intermediate with an alkylthiol. The product is then purified using column chromatography. NDTM can be characterized using a variety of techniques, including NMR, mass spectrometry, and HPLC.

Analytical Methods:

NDTM can be quantitated using HPLC or UV-vis spectrophotometry. HPLC is a useful tool for analyzing the purity of NDTM, while UV-vis spectrophotometry can be used to measure the concentration of NDTM in solution.

Biological Properties:

NDTM has been shown to be an effective detergent for solubilizing membrane proteins, making it useful in a variety of biological research applications. It has also been used as a medium for the crystallization of water-soluble proteins. NDTM is non-toxic to cells and has been used in a variety of different cell-based assays.

Toxicity and Safety in Scientific Experiments:

NDTM is generally considered to be safe for use in scientific experiments. It is non-toxic to cells and has a low toxicity profile. However, researchers must take care to use appropriate safety measures when handling NDTM, as it can be irritating to the eyes and skin.

Applications in Scientific Experiments:

NDTM has a wide range of applications in scientific research. It is commonly used to solubilize membrane proteins for structural and functional studies. It has also been used as a medium for the crystallization of water-soluble proteins. In addition, NDTM has been used in the development of new vaccines and drug delivery systems.

Current State of Research:

Research on NDTM continues to expand, with new studies exploring its potential applications in a variety of different fields. Recent studies have focused on its use in the development of new vaccines and drug delivery systems, as well as its potential as a tool for studying the structure and function of membrane proteins.

Potential Implications in Various Fields of Research and Industry:

NDTM has numerous potential applications in a variety of different fields, including medicine, biotechnology, and pharmaceuticals. It has been used in the development of new vaccines and drug delivery systems, and its unique properties make it a valuable tool for studying the structure and function of membrane proteins.

Limitations and Future Directions:

While NDTM has many potential applications, there are also limitations to its use. One significant limitation is its cost, which may limit its use in large-scale experiments. In addition, its utility is limited to the study of membrane proteins, and it may not be useful for the study of other types of proteins or molecules. Future research in this area should focus on exploring new applications for NDTM and on developing more cost-effective methods for its synthesis and use.

Future Directions:

• Exploring new applications for NDTM in biotechnology, medicine, and pharmaceuticals

• Developing more cost-effective methods for the synthesis and use of NDTM

• Investigating the potential of NDTM in the study of other types of proteins and molecules

• Identifying new potential applications for NDTM in industrial processes and applications, such as in the production of new materials and chemicals

• Combining NDTM with other surfactants to create new formulations with unique properties

• Exploring the use of NDTM in the development of new diagnostic tools and assays.