Octyl 2-acetamido-2-deoxy-b-D-glucopyranoside

辛基-2-乙酰氨基-2-脱氧-β-D-葡萄糖苷

Octyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a lyotropic liquid that is flammable. It can be used to prepare potassium hydride, which is an organometallic. Potassium hydride reacts with organic acids and atorvastatin to produce argon gas. This reaction can be done in the presence of chlorine gas instead of air. Preparative methods for octyl 2-acetamido-2-deoxy-b-D-glucopyranoside include heating it in a vacuum or under argon gas and then reacting it with potassium tetrachlorocobaltate in water or ethanol solution.

Octyl 2-Acetamido-2-Deoxy-b-D-Glucopyranoside (ODG) is a non-ionic surfactant with hydrophobic octyl and hydrophilic oligosaccharide groups. It is widely used in biochemistry and biophysics research as a mild and effective detergent for the solubilization and purification of membrane proteins.

Physical and Chemical Properties:

ODG is a white to off-white crystalline powder with a molecular weight of 333.42 g/mol. It is soluble in water and organic solvents such as methanol, ethanol, and dimethyl sulfoxide. ODG has a critical micelle concentration (CMC) of 0.013 mM in water at 25°C, which means that it forms micelles at a low concentration in solution.

Synthesis and Characterization:

ODG can be synthesized from octyl glucoside and N-acetylglucosamine using chemical or enzymatic methods. The product can be purified by column chromatography and characterized by various analytical methods such as nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and high-performance liquid chromatography (HPLC).

Analytical Methods:

Various analytical methods can be used to measure the concentration and purity of ODG, such as UV spectrophotometry, HPLC, and gas chromatography (GC). The CMC of ODG can be determined by surface tension measurements or fluorescence spectroscopy.

Biological Properties:

ODG has been shown to be a mild and effective detergent for the solubilization and purification of membrane proteins, especially those with low thermostability and low expression levels. It has been used in a wide range of biological research, including structural biology, enzymology, and proteomics.

Toxicity and Safety in Scientific Experiments:

ODG has low toxicity and has been approved for use in various scientific experiments. However, it should be handled with care and proper protective equipment should be used when working with it in the laboratory.

Applications in Scientific Experiments:

ODG has a wide range of applications in scientific experiments, including the solubilization and purification of integral membrane proteins, membrane-bound enzymes, and receptor proteins. It has also been used in the crystallization, biophysical characterization, and functional analysis of these proteins.

Current State of Research:

ODG has been widely used in biochemical and biophysical research for over two decades. Many studies have been conducted to investigate its potential applications in various fields of research and industry.

Potential Implications in Various Fields of Research and Industry:

ODG has potential implications in various fields of research and industry, including pharmaceuticals, biotechnology, and food science. Its effectiveness as a detergent for membrane proteins and its low toxicity make it a promising candidate for drug discovery and development. It can also be used in the development of novel biotechnological processes and food products.

Limitations and Future Directions:

One limitation of ODG is its high cost compared to other detergents. Future research could focus on the development of more efficient and cost-effective methods of production. Additionally, further studies can be conducted to investigate the potential use of ODG in other fields, such as nanotechnology and environmental science.

Future Directions:

1. Development of more efficient and cost-effective methods of production.

2. Investigation of the potential use of ODG in the field of nanotechnology.

3. Study of the effects of ODG on cell membranes and their potential applications in drug delivery.

4. Investigation of the potential use of ODG as a solubilizing agent for hydrophobic compounds.

5. Development of new analytical methods for the detection and quantification of ODG.

6. Study of the interactions between ODG and proteins and their potential applications in drug discovery and development.

7. Investigation of the potential use of ODG in the development of biosensors and diagnostic tools.

8. Study of the effects of ODG on the environment and the development of eco-friendly formulations.